Description:

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

Features&advantages:

-Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

-The sample demand is small, as low as 10 cells can be tested.

-High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

-DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

-Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Kit application:

-Scope of application: cultured cells.

-RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

-The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

Diagram:

Storage and Shelf life:

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

-Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

-Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

Precautions: (Be sure to read the precautions carefully before using the kit)

- Pay attention to the operation method of the experiment to avoid cross-contamination between samples.

- Pay attention to the cleanliness of the experimental environment and utensils to avoid RNase contamination and RNA degradation.

- Take fresh or well-preserved cell samples, never use repeated freeze-thawed cell samples.

- 5× Direct RT Mix, 2× Direct qPCR Mix-SYBR should avoid repeated freeze-thaw, otherwise it will affect reverse transcription and PCR efficiency.

- Cell lysis system please prepare freshly, that is, ready to use.